Anti-Tec antibody [Y398]
| Name | Anti-Tec antibody [Y398] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32368 |
| Prices | $99.00, $387.00 |
| Sizes | 10 µl, 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | Y398 |
| Applications | WB IHC-P ICC/IF FC IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues in the PH domain of human Tec |
| Description | Rabbit Monoclonal |
| Gene | TEC |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing Jurkat cells stained with ab32368 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32368, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Anti-Tec antibody [Y398] (ab32368) at 1/10000 dilution + Jurkat cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/9689_(ab32368-1.JPG)
Anti-Tec antibody [Y398] (ab32368) at 1/10000 dilution + Jurkat cell lysate
ab32368, at a 1/50 dilution,staining human ovary carcinoma by immunohistochemistry, Paraffin embedded tissue.ab32368, at a 1/50 dilution,staining human ovary carcinoma by immunohistochemistry, Paraffin embedded tissue.
Product References
The kinase Btk negatively regulates the production of reactive oxygen species and - The kinase Btk negatively regulates the production of reactive oxygen species and
Honda F, Kano H, Kanegane H, Nonoyama S, Kim ES, Lee SK, Takagi M, Mizutani S, Morio T. Nat Immunol. 2012 Feb 26;13(4):369-78.
Essential role of Stat3 in PI3K-induced oncogenic transformation. - Essential role of Stat3 in PI3K-induced oncogenic transformation.
Hart JR, Liao L, Yates JR 3rd, Vogt PK. Proc Natl Acad Sci U S A. 2011 Aug 9;108(32):13247-52. doi: