All lanes : Anti-TECK antibody [EPR12388(2)] (ab200343) at 1/10000 dilutionLane 1 : Human thymus lysateLane 2 : Human fetal lung lysateLane 3 : Human fetal brain lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling TECK using ab200343 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab200343 and secondary antibody only.Note: Cytoplasm staining on human thymus tissue was observed.
Immunohistochemical analysis of paraffin-embedded human small intestine tissue labeling TECK using ab200343 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab200343 and secondary antibody only.Note: Cytoplasm staining on human small intestine tissue was observed.
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TECK using ab200343 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab200343 and secondary antibody only.Note: No staining on human colon tissue was observed.