Anti-Tenascin C antibody [EPR4219]

• Supplier: Abcam
• Catalog: ab108930
• Prices: $395.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4219
• Applications: WB IHC-P ICC/IF FC IHC-F
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues in Human Tenascin C
• Description: Rabbit Monoclonal
• Gene: TNC
• Conjugate: Unconjugated

Product images

Overlay histogram showing SH-SY5Y cells stained with ab108930 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108930, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution + Human fetal brain lysate at 10 µg

Product References

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Noncanonical transforming growth factor beta (TGFbeta) signaling in cranial - Noncanonical transforming growth factor beta (TGFbeta) signaling in cranial

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