Anti-Tet2 antibody
| Name | Anti-Tet2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab94580 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IP ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Human, Marmoset, Dog |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Tet2 |
| Description | Rabbit Polyclonal |
| Gene | TET2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-Tet2 antibody (ab94580) at 1 µg/ml (blocked with 5% BSA)Lane 2 : Anti-Tet2 antibody (ab94580) at 1 µg/ml (blocked with 5% milk)Lane 3 : Anti-Tet2 antibody (ab94580) at 1 µg/ml (blocked with 2% milk)Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab94580 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab94580 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa pfa fixed (4%, 10 minutes) cell types at 10ug/ml.
Anti-Tet2 antibody (ab94580) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
PGC7 suppresses TET3 for protecting DNA methylation. - PGC7 suppresses TET3 for protecting DNA methylation.
Bian C, Yu X. Nucleic Acids Res. 2014 Mar;42(5):2893-905.
Modulation of TET2 expression and 5-methylcytosine oxidation by the CXXC domain - Modulation of TET2 expression and 5-methylcytosine oxidation by the CXXC domain
Ko M, An J, Bandukwala HS, Chavez L, Aijo T, Pastor WA, Segal MF, Li H, Koh KP, Lahdesmaki H, Hogan PG, Aravind L, Rao A. Nature. 2013 May 2;497(7447):122-6.
Meta-analysis of IDH-mutant cancers identifies EBF1 as an interaction partner for - Meta-analysis of IDH-mutant cancers identifies EBF1 as an interaction partner for
Guilhamon P, Eskandarpour M, Halai D, Wilson GA, Feber A, Teschendorff AE, Gomez V, Hergovich A, Tirabosco R, Fernanda Amary M, Baumhoer D, Jundt G, Ross MT, Flanagan AM, Beck S. Nat Commun. 2013;4:2166.
Ten-eleven translocation-2 (TET2) is a master regulator of smooth muscle cell - Ten-eleven translocation-2 (TET2) is a master regulator of smooth muscle cell
Liu R, Jin Y, Tang WH, Qin L, Zhang X, Tellides G, Hwa J, Yu J, Martin KA. Circulation. 2013 Oct 29;128(18):2047-57.