Lanes 1 - 4 : anti-Flag antibodyLanes 5 - 8 : Anti-Tet2 antibody (ab135087)Lane 1 : Empty vector transfected HEK293T cellsLane 2 : Flag-tagged mouse Tet1 transfected HEK293T cellsLane 3 : Flag-tagged mouse Tet2 transfected HEK293T cellsLane 4 : Flag-tagged mouse Tet3 transfected HEK293T cellsLane 5 : Empty vector transfected HEK293T cellsLane 6 : Flag-tagged mouse Tet1 transfected HEK293T cellsLane 7 : Flag-tagged mouse Tet2 transfected HEK293T cellsLane 8 : Flag-tagged mouse Tet3 transfected HEK293T cells