Detection of TET3 in Immunoprecipitate of TET3-transfected 293T cells. Lane 1: Transfected 293T cell lysate. Lane 2: Control with 2 µg of preimmune rabbit IgG Lane 3: Immunoprecipitate of TET3-transfected 293T cells using ab153724 at 2 µg. Detection using ab153724 at 1/1000 dilution.
All lanes : Anti-TET3 antibody - C-terminal (ab153724) at 1/5000 dilutionLane 1 : 293T whole cell lysateLane 2 : Human TET3-transfected 293T whole cell lysate with DDDDK tag Lysates/proteins at 30 µg per lane.
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling TET3 with ab153724 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.