All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/2000 dilution (purified)Lane 1 : U87-MG cell lysateLane 2 : Human placenta tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Anti-TFPI antibody [EPR7941] (ab134151) at 1/10000 dilution (purified) + HepG2 cell lysate at 20 µgSecondaryHRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/1000 dilution (unpurified)Lane 1 : HepG2 cell lysateLane 2 : U87-MG cell lysateLane 3 : Caco-2 cell lysateLane 4 : Human placenta tissue lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG at 1/2000 dilution