All lanes : Anti-Thimet Oligopeptidase antibody - Catalytic domain (ab59487) at 1/1000 dilutionLane 1 : Recombinant Human THOP1 (Thimet Oligopeptidase) at 0.08 µgLane 2 : Recombinant Human THOP1 (Thimet Oligopeptidase) at 0.04 µgLane 3 : Recombinant Human THOP1 (Thimet Oligopeptidase) at 0.02 µgLane 4 : Recombinant Human THOP1 (Thimet Oligopeptidase) at 0.01 µgLane 5 : Recombinant Human THOP1 (Thimet Oligopeptidase) at 0.005 µg
ab59487 used in Immunoprecipitation and Western Blot.Immunoprecipitation of NLN (EC 3.4.24.16), Thimet Oligopeptidase (thimet OP; EC 3.4.24.15), a-Adducin, and collapsin response mediator protein 2 (CRMP2) from photoradiolabeled P10 mouse forebrain membrane preparations. Immunoprecipitation of each protein was carried out from parallel nonspecific (NS) and total (Tot) photoradiolabeled groups.a, equal amounts of NS and total sample of each protein immunoprecipitate were used to count the radioactive signal. Bck, background radioactivity; ***, p < 0.001 versus all other groups; n = 3–4).b–e, representative Western blot confirmation of successful immunoprecipitation of each candidate protein from NS and total samples.