Anti-Thioredoxin / TRX antibody
| Name | Anti-Thioredoxin / TRX antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab86255 |
| Prices | $395.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human, Sheep, Horse, Bovine |
| Antigen | Recombinant human protein purified from E |
| Description | Rabbit Polyclonal |
| Gene | TXN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Thioredoxin / TRX staining in human ovary formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86255, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab86255 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86255, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Overexpression of thioredoxin-binding protein 2 increases oxidation sensitivity - Overexpression of thioredoxin-binding protein 2 increases oxidation sensitivity
Yu Y, Xing K, Badamas R, Kuszynski CA, Wu H, Lou MF. Free Radic Biol Med. 2013 Apr;57:92-104. doi:
Evaluation of the salivary proteome as a surrogate tissue for systems biology - Evaluation of the salivary proteome as a surrogate tissue for systems biology
Harden CJ, Perez-Carrion K, Babakordi Z, Plummer SF, Hepburn N, Barker ME, Wright PC, Evans CA, Corfe BM. J Proteomics. 2012 Jun 6;75(10):2916-23.