TXN

Gene Symbol TXN
Entrez Gene 7295
Alt Symbol TRDX, TRX, TRX1
Species Human
Gene Type protein-coding
Description thioredoxin
Other Description ADF|ATL-derived factor|SASP|TXN delta 3|surface-associated sulphydryl protein|thioredoxin delta 3
Swissprots Q96KI3 O60744 B1ALW1 Q9UDG5 P10599 Q53X69
Accessions AAN33187 CAA38410 EAW59059 EAW59060 P10599 AF065241 AAC17430 AF085844 AF276919 AAF86466 AF313911 AAG34699 AK289508 BAF82197 AW160756 AY004872 AAF87085 BC003377 AAH03377 BC054866 AAH54866 BQ774337 BX647247 CR407665 CAG28593 D28376 BAA05742 J04026 AAA74596 JQ313905 AFH41799 X77584 CAA54687 NM_001244938 NP_001231867 NM_003329 NP_003320
Function Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
Subcellular Location Nucleus. Cytoplasm. Secreted. Note=Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.

Thioredoxin 1 (C63C6) Rabbit mAb - 2429 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C63C6
Western blot analysis of extracts from various cell types using Thioredoxin 1 (C63C6) Rabbit mAb.

Thioredoxin 1 Antibody (Mouse/Rat Preferred) - 2298 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines, using Thioredoxin 1 Antibody (Mouse/Rat Preferred).

Thioredoxin 1 Antibody (Human Specific) - 2285 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines, using Thioredoxin 1 Antibody (Human Specific).

Trx (D-4) - sc-271281 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Trx (D-4): sc-271281. Western blot analysis of Trx expression in HeLa (A) and BJAB (B) whole cell lysates.

Trx (FL-105) - sc-20146 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Trx (FL-105): sc-20146. Western blot analysis of Trx expression in THP-1 (A), JEG-3 (B) and HeLa (C) whole cell lysates.

Trx (N-20) - sc-18215 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Trx (N-20): sc-18215. Western blot analysis of Trx expression in AML-193 whole cell lysate.

Trx (A-5) - sc-166393 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Trx (A-5): sc-166393. Western blot analysis of Trx expression in THP-1 (A), AML-193 (B) and BJAB (C) whole cell lysates.

Trx (2) - sc-101512 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Trx (2): sc-101512. Western blot analysis of human recombinant Trx.

Trx (1H6H6) - sc-81155 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Trx (1H6H6): sc-81155. Western blot analysis of human recombinant Thioredoxin.

Trx (3A1) - sc-58440 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Trx (3A1): sc-58440. Western blot analysis of Trx expression in non-transfected 293: sc-110760 (A), human Trx transfected 293: sc-110552 (B), THP-1 (C) and AML-193 (D) whole cell lysates.

Anti-Thioredoxin / TRX antibody - ab26320 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Thioredoxin / TRX was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Thioredoxin / TRX and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab26320.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 12kDa; Thioredoxin / TRX

Anti-Thioredoxin / TRX antibody - ab86255 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC image of Thioredoxin / TRX staining in human ovary formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86255, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Thioredoxin / TRX antibody - ab185329 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Thioredoxin / TRX antibody (ab185329) at 1/600 dilutionLane 1 : Hela cell tissue lysateLane 2 : MCF7 cell tissue lysateLane 3 : Human liver cancer tissue lysateLysates/proteins at 40 µg per lane.SecondaryGoat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

Anti-Thioredoxin / TRX antibody - ab26928 from Abcam

Host Chicken
Clonality Polyclonal
Anti-Thioredoxin / TRX antibody (ab26928) at 1/2000 dilution + 200 ng human Thioredoxin proteinSecondaryGoat anti IgY HRP at 1/10000 dilutiondeveloped using the ECL technique

Anti-Thioredoxin / TRX antibody - ab115758 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Thioredoxin / TRX antibody (ab115758) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysate

Anti-Thioredoxin / TRX antibody - ab57675 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Thioredoxin / TRX antibody - ab57676 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Thioredoxin / TRX antibody [2B1] - ab1754 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2B1
Image from Codreanu SG et al, Mol Cell Proteomics. 2009 Apr;8(4):670-80. Epub 2008 Dec 2, Fig 4. DOI .1074/mcp.M800070-MCP200

Anti-Thioredoxin / TRX antibody [3A1] - ab16965 from Abcam

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3A1
All lanes : Anti-Thioredoxin / TRX antibody [3A1] (ab16965) at 1/2000 dilutionLane 1 : Cell lysates prepared from Hela cellsLane 2 : Cell lysates prepared from Hela cellsLysates/proteins at 20 µg per lane.

Anti-Thioredoxin / TRX antibody [8A1] - ab16845 from Abcam

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 8A1
Immunoprecipitation analysis of 293T cell lysates using ab16845.Lane 1: Input.Lane 2: Precipitates.

Anti-Thioredoxin / TRX antibody [EPR6110] - ab133524 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6110
All lanes : Anti-Thioredoxin / TRX antibody [EPR6110] (ab133524) at 1/10000 dilutionLane 1 : MCF7 cell lysateLane 2 : THP1 cell lysateLane 3 : HeLa cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Anti-Thioredoxin / TRX antibody [EPR6111] - ab109385 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6111
Overlay histogram showing HeLa cells stained with ab109385 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109385, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-TXN / Thioredoxin / TRX Antibody - LS-C137600 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-TXN / Thioredoxin / TRX Antibody (FITC) - LS-C183348 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-TXN / Thioredoxin / TRX Antibody (Biotin) - LS-C183349 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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