![Overlay histogram showing HeLa cells stained with ab16965 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16965, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/10529_Thioredoxin-TRX-Primary-antibodies-ab16965-1.jpg)
Overlay histogram showing HeLa cells stained with ab16965 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16965, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-Thioredoxin / TRX antibody [3A1] (ab16965) at 1/2000 dilutionLane 1 : Cell lysates prepared from Hela cellsLane 2 : Cell lysates prepared from Hela cellsLysates/proteins at 20 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/10528_ab16965_1.JPG)
All lanes : Anti-Thioredoxin / TRX antibody [3A1] (ab16965) at 1/2000 dilutionLane 1 : Cell lysates prepared from Hela cellsLane 2 : Cell lysates prepared from Hela cellsLysates/proteins at 20 µg per lane.