Anti-Thrombomodulin antibody [PBS-01]
| Name | Anti-Thrombomodulin antibody [PBS-01] |
|---|---|
| Supplier | Abcam |
| Catalog | ab6980 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | PBS-01 |
| Applications | ELISA WB FC IHC-P |
| Species Reactivities | Rabbit, Human |
| Antigen | Recombinant full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | THBD |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab6980 staining human normal lung endothelium. Staining is localized to the membrane.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
![Overlay histogram showing THP1 cells stained with ab6980 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6980, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/10655_Thrombomodulin-Primary-antibodies-ab6980-7.jpg)
Overlay histogram showing THP1 cells stained with ab6980 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6980, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Anti-Thrombomodulin antibody [PBS-01] (ab6980) at 1/100 dilution + whole cell lysate prepared from human aortic endothelial cells at 30 µgSecondaryHRP conjugated anti-mouse IgG at 1/4000 dilutiondeveloped using the ECL techniqueObserved band size : 95 kDa (why is the actual band size different from the predicted?)Exposure time : 10 minutesImage courtesy of an anonymous Abreview.36kDa band is GAPDH in the same lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/10656_Thrombomodulin-Primary-antibodies-ab6980-6.jpg)
Anti-Thrombomodulin antibody [PBS-01] (ab6980) at 1/100 dilution + whole cell lysate prepared from human aortic endothelial cells at 30 µgSecondaryHRP conjugated anti-mouse IgG at 1/4000 dilutiondeveloped using the ECL techniqueObserved band size : 95 kDa (why is the actual band size different from the predicted?)Exposure time : 10 minutesImage courtesy of an anonymous Abreview.36kDa band is GAPDH in the same lane.
Product References
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Thrombomodulin is silenced in malignant mesothelioma by a poly(ADP-ribose) - Thrombomodulin is silenced in malignant mesothelioma by a poly(ADP-ribose)
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Flow-Dependent Regulation of Kruppel-Like Factor 2 Is Mediated by MicroRNA-92a. - Flow-Dependent Regulation of Kruppel-Like Factor 2 Is Mediated by MicroRNA-92a.
Wu W, Xiao H, Laguna-Fernandez A, Villarreal G Jr, Wang KC, Geary GG, Zhang Y, Wang WC, Huang HD, Zhou J, Li YS, Chien S, Garcia-Cardena G, Shyy JY. Circulation. 2011 Aug 2;124(5):633-41.
Vascular lesions and s-thrombomodulin concentrations from auricular arteries of - Vascular lesions and s-thrombomodulin concentrations from auricular arteries of
Zachary JF, Blue JP, Miller RJ, O'Brien WD Jr. Ultrasound Med Biol. 2006 Nov;32(11):1781-91.