Immunofluorescent analysis of Jurkat cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Thrombopoietin with ab196026 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).Note: Cytoplasm staining on Jurkat cell line was observed.
Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling Thrombopoietin with ab196026 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).Note: Cytoplasm staining on human bone marrow tissue was observed.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Thrombopoietin with ab196026 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).Note: Cytoplasm staining on human liver tissue was observed.
Anti-Thrombopoietin antibody [EPR14948] (ab196026) at 1/5000 dilution + Thrombopoietin recombinant protein at 0.01 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-Thrombopoietin antibody [EPR14948] (ab196026) at 1/1000 dilution + Human fetal liver at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution