Anti-Thymidylate Synthase antibody
| Name | Anti-Thymidylate Synthase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58287 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | FC WB IHC-P ICC/IF ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC , corresponding to amino acids 111-211 of Human Thymidylate Synthase Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | TYMS |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Thymidylate Synthase antibody (ab58287) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
ICC/IF image of ab58287 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58287, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Thymidylate Synthase antibody (ab58287) at 1 µg/mlLane 1 : Human Thymidylate Synthase full length protein (ab95378) at 0.1 µgLane 2 : Human Thymidylate Synthase full length protein (ab95378) at 0.01 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 10 seconds
![Overlay histogram showing HeLa cells stained with ab58287 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58287, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/10836_Thymidylate-Synthase-Primary-antibodies-ab58287-5.jpg)
Overlay histogram showing HeLa cells stained with ab58287 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58287, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Variation in drug sensitivity of malignant mesothelioma cell lines with - Variation in drug sensitivity of malignant mesothelioma cell lines with
Szulkin A, Nilsonne G, Mundt F, Wasik AM, Souri P, Hjerpe A, Dobra K. PLoS One. 2013 Jun 20;8(6):e65903.
Identification of genes conferring resistance to 5-fluorouracil. - Identification of genes conferring resistance to 5-fluorouracil.
Yoo BK, Gredler R, Vozhilla N, Su ZZ, Chen D, Forcier T, Shah K, Saxena U, Hansen U, Fisher PB, Sarkar D. Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12938-43. doi: