TYMS

Gene Symbol TYMS
Entrez Gene 7298
Alt Symbol HST422, TMS, TS
Species Human
Gene Type protein-coding
Description thymidylate synthetase
Other Description TSase|thymidylate synthase
Swissprots Q8WYK3 P04818 Q8WYK4
Accessions AAD43903 AAF66448 BAA00404 BAA00472 CBN68109 EAX01716 EAX01717 EAX01718 EAX01719 EAX01720 EAX01721 EAX01722 P04818 AB062290 BAB93473 AB077207 BAB83676 AB077208 BAB83677 AK223372 BAD97092 AK292620 BAF85309 AK314140 BAG36830 BC002567 AAH02567 BC013919 AAH13919 BC018858 BC083512 AAH83512 BT006811 AAP35457 DQ892149 ABM83075 X02308 CAA26178 NM_001071 NP_001062
Function Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. {ECO:0000269|PubMed:21876188}.
Subcellular Location Nucleus {ECO:0000269|PubMed:21876188}. Cytoplasm {ECO:0000269|PubMed:21876188}. Mitochondrion {ECO:0000269|PubMed:21876188}. Mitochondrion matrix {ECO:0000269|PubMed:21876188}. Mitochondrion inner membrane {ECO:0000269|PubMed:21876188}.
Top Pathways Pyrimidine metabolism, One carbon pool by folate

Thymidylate Synthase (D5B3) XP ® Rabbit mAb - 9045 from Cell Signaling Technology

Prices $109.00, $282.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5B3
Western blot analysis of extracts from SK-BR-3 and HCC827 cells, untreated or treated with 5-FU (1 μM) or lapatinib (1 μM) for 24 hr, using Thymidylate Synthase (D5B3) XP ® Rabbit mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).

Thymidylate Synthase (TS106) Mouse mAb - 5653 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone TS106
Western blot analysis of extracts from various cell lines using Thymidylate Synthase (TS106) Mouse mAb.

Thymidylate Synthase (D26G11) Rabbit mAb - 5449 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D26G11
Western blot analysis of extracts from various cell lines using Thymidylate Synthase (D26G11) Rabbit mAb.

Thymidylate Synthase Antibody - 3766 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using Thymidylate Synthase Antibody.

TS (C-5) - sc-390945 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TS (C-5): sc-390945. Western blot analysis of TS expression in HeLa (A), HL-60 (B), Jurkat (C), IMR-32 (D) and SH-SY5Y (E) whole cell lysates.

TS (D-2) - sc-393037 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TS (D-2): sc-393037. Western blot analysis of TS expression in HeLa (A), SH-SY5Y (B), Jurkat (C) and IMR-32 (D) whole cell lysates.

TS (F-7) - sc-376161 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TS (F-7): sc-376161. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.

TS (I-13) - sc-48105 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TS (I-13): sc-48105. Western blot analysis of TS expression in non-transfected: sc-117752 (A) and human TS transfected: sc-170183 (B) 293T whole cell lysates.

TS (TS 106) - sc-33679 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TS (TS 106): sc-33679. Western blot analysis of TS expression in HeLa (A), IMR-32 (B), SH-SY5Y (C) and HL-60 (D) whole cell lysates.

TS (SPM453) - sc-56492 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TS (SPM453): sc-56492. Western blot analysis of TS expression in non-transfected: sc-117752 (A) and human TS transfected: sc-170183 (B) 293T whole cell lysates.

Anti-Thymidylate Synthase antibody - ab155795 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Thymidylate Synthase antibody (ab155795) at 1/1000 dilutionLane 1 : Jurkat whole cell lysateLane 2 : Raji whole cell lysateLane 3 : THP1 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Thymidylate Synthase antibody [2C3] - ab170422 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2C3
All lanes : Anti-Thymidylate Synthase antibody [2C3] (ab170422) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : HEK293T cells transfected with pCMV6-ENTRY Thymidylate SynthaseLysates/proteins at 5 µg per lane.

Anti-Thymidylate Synthase antibody [3A7.A11] - ab990 from Abcam

Prices $372.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3A7.A11

Anti-Thymidylate Synthase antibody [EPR4545] - ab108995 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4545
All lanes : Anti-Thymidylate Synthase antibody [EPR4545] (ab108995) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : HL60 cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 10 µg per lane.Performed under reducing conditions.

Anti-Thymidylate Synthase antibody [EPR4545] (Alexa Fluor® 488) - ab197898 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4545
Overlay histogram showing HeLa cells stained with ab197898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197898, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Thymidylate Synthase antibody [EPR4545] (Alexa Fluor® 647) - ab197899 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4545
Overlay histogram showing HeLa cells stained with ab197899 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197899, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.

Anti-Thymidylate Synthase antibody [EPR4545] (HRP) - ab198599 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4545
All lanes : Anti-Thymidylate Synthase antibody [EPR4545] (HRP) (ab198599) at 1/5000 dilutionLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : HL60 (Human promyelocytic leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Thymidylate Synthase antibody [SP112] - ab111153 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone SP112
ab111153 at 1/100 dilution staining Thymidylate Synthase in Formalin-fixed, paraffin-embedded Human colon adenocarcinoma tissue.

Anti-Thymidylate Synthase antibody [TMS715] - ab199009 from Abcam

Prices $373.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone TMS715

Anti-Thymidylate Synthase antibody [TS 106] - ab3145 from Abcam

Prices $395.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone TS 106
Anti-Thymidylate Synthase antibody [TS 106] (ab3145) at 1 µg/ml + HL60 whole cell lysate at 20 µgSecondaryHRP conjugated sheep F(ab') anti-mousedeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Thymidylate Synthase antibody [TS106 + TMS715] - ab199003 from Abcam

Prices $359.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone TS106 + TMS715

Anti-Thymidylate Synthase antibody - ab7398 from Abcam

Prices $369.00
Sizes 1 mg
Host Sheep
Clonality Polyclonal

Anti-Thymidylate Synthase antibody - ab58287 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Thymidylate Synthase antibody (ab58287) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.

Anti-Thymidylate Synthase antibody - ab34836 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab34836 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Thymidylate Synthase antibody - ab97510 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Thymidylate Synthase antibody (ab97510) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
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