Western blot analysis of extracts from GDM-1 cells, serum-starved overnight and untreated (-) or treated with Human Macrophage Colony Stimulating Factor (hM-CSF) #8929 (100 ng/ml, 5 min; +), using Phospho-M-CSF Receptor (Tyr708) (D5F4Y) Rabbit mAb (upper) and M-CSF Receptor Antibody #3152 (lower).
Immunoprecipitation of phospho-M-CSF receptor (Tyr708) from serum-starved GDM-1 cell extracts treated with Human Macrophage Colony Stimulating Factor (hM-CSF) #8929 (100 ng/ml, 5 min; +), using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-M-CSF Receptor (Tyr708) (D5F4Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-M-CSF Receptor (Tyr708) (D5F4Y) Rabbit mAb.