Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade
| Name | Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab5622 |
| Prices | $381.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF EMSA ELISA ChIP IHC-P ICC/IF WB |
| Species Reactivities | Mouse, Rat, Rabbit, Human |
| Antigen | Synthetic peptide corresponding to Human Thyroid Hormone Receptor beta aa 62-81 |
| Description | Rabbit Polyclonal |
| Gene | THRB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Rat thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Human thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Product References
Reduced expression of thyroid hormone receptors and beta-adrenergic receptors in - Reduced expression of thyroid hormone receptors and beta-adrenergic receptors in
Modesti PA, Marchetta M, Gamberi T, Lucchese G, Maccherini M, Chiavarelli M, Modesti A. Biochem Pharmacol. 2008 Feb 15;75(4):900-6. Epub 2007 Oct 16.
Liver proteome analysis of adaptive response in rat immediately after partial - Liver proteome analysis of adaptive response in rat immediately after partial
Sun Y, Deng X, Li W, Yan Y, Wei H, Jiang Y, He F. Proteomics. 2007 Dec;7(23):4398-407.