Anti-TIA1 antibody
| Name | Anti-TIA1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab40693 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Monkey |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human TIA1 |
| Description | Rabbit Polyclonal |
| Gene | TIA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-TIA1 antibody (ab40693) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab40693 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40693, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab40693 staining TIA1 in human lymphoma tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with peroxidase for 5 minutes followed by a protein block for 10 minutes at 20°C . Antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody 1/2000 for 45 minutes at 20°C. An HRP-conjugated Goat polycolonal to rabbit IgG was used as secondary antibody. The image shows clean predominantly nuclear staining. See Abreview
Product References
A murine retrovirus co-Opts YB-1, a translational regulator and stress - A murine retrovirus co-Opts YB-1, a translational regulator and stress
Bann DV, Beyer AR, Parent LJ. J Virol. 2014 Apr;88(8):4434-50.
ALS mutant FUS disrupts nuclear localization and sequesters wild-type FUS within - ALS mutant FUS disrupts nuclear localization and sequesters wild-type FUS within
Vance C, Scotter EL, Nishimura AL, Troakes C, Mitchell JC, Kathe C, Urwin H, Manser C, Miller CC, Hortobagyi T, Dragunow M, Rogelj B, Shaw CE. Hum Mol Genet. 2013 Jul 1;22(13):2676-88.
PKCalpha binds G3BP2 and regulates stress granule formation following cellular - PKCalpha binds G3BP2 and regulates stress granule formation following cellular
Kobayashi T, Winslow S, Sunesson L, Hellman U, Larsson C. PLoS One. 2012;7(4):e35820.
Urinary peptidome may predict renal function decline in type 1 diabetes and - Urinary peptidome may predict renal function decline in type 1 diabetes and
Merchant ML, Perkins BA, Boratyn GM, Ficociello LH, Wilkey DW, Barati MT, Bertram CC, Page GP, Rovin BH, Warram JH, Krolewski AS, Klein JB. J Am Soc Nephrol. 2009 Sep;20(9):2065-74.