All lanes : Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/10000 dilutionLane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysateLane 2 : 293 (Human embryonic kidney) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-TIAM2 antibody [EPR16838] (ab199426) at 2000 cells + Human fetal brain lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/1000 dilutionLane 1 : Rat brain lysate at 10 µgLane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µgLane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 mg/mlLane 4 : NIH/3T3 (mouse embryo fibroblast cells) whole cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/1000 dilution + C6 (rat glial tumor cells) whole cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling TIAM2 with ab199426 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human gliocytoma tissue is observed. Counter stained with Hematoxylin.Negative control: Used only secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TIAM2 with ab199426 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human testis tissue is observed. Counter stained with Hematoxylin.Negative control: Used only secondary antibody.
Flow cytometric analysis of 2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling U TIAM2 with ab199426 at 1/450 dilution (red). The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is Rabbit monoclonal IgG (black) and the cell without incubation with primary antibody and secondary antibody is blue.