Anti-TIGAR antibody
| Name | Anti-TIGAR antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab37910 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human TIGAR |
| Description | Rabbit Polyclonal |
| Gene | TIGAR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-TIGAR antibody (ab37910) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab37910 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37910, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab37910 staining human adrenal tissue sections by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 25°C. A biotinylated goat anti-rabbit antibody diluted 1/400 was used as the secondary.See Abreview
ICC/IF image of ab37910 stained HeLa cells. The cells were fixed with 100% methanol (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37910 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 1µg/ml.
Product References
Chronic cerebral hypoperfusion causes decrease of O-GlcNAcylation, - Chronic cerebral hypoperfusion causes decrease of O-GlcNAcylation,
Zhao Y, Gu JH, Dai CL, Liu Q, Iqbal K, Liu F, Gong CX. Front Aging Neurosci. 2014 Feb 10;6:10.
TIGAR has a dual role in cancer cell survival through regulating apoptosis and - TIGAR has a dual role in cancer cell survival through regulating apoptosis and
Xie JM, Li B, Yu HP, Gao QG, Li W, Wu HR, Qin ZH. Cancer Res. 2014 Sep 15;74(18):5127-38.
Autophagy in idiopathic pulmonary fibrosis. - Autophagy in idiopathic pulmonary fibrosis.
Patel AS, Lin L, Geyer A, Haspel JA, An CH, Cao J, Rosas IO, Morse D. PLoS One. 2012;7(7):e41394.
Inhibition of the MUC1-C oncoprotein induces multiple myeloma cell death by - Inhibition of the MUC1-C oncoprotein induces multiple myeloma cell death by
Yin L, Kosugi M, Kufe D. Blood. 2012 Jan 19;119(3):810-6.
The histone deacetylase Sirt6 regulates glucose homeostasis via Hif1alpha. - The histone deacetylase Sirt6 regulates glucose homeostasis via Hif1alpha.
Zhong L, D'Urso A, Toiber D, Sebastian C, Henry RE, Vadysirisack DD, Guimaraes A, Marinelli B, Wikstrom JD, Nir T, Clish CB, Vaitheesvaran B, Iliopoulos O, Kurland I, Dor Y, Weissleder R, Shirihai OS, Ellisen LW, Espinosa JM, Mostoslavsky R. Cell. 2010 Jan 22;140(2):280-93.
Autophagy in cancer associated fibroblasts promotes tumor cell survival: Role of - Autophagy in cancer associated fibroblasts promotes tumor cell survival: Role of
Martinez-Outschoorn UE, Trimmer C, Lin Z, Whitaker-Menezes D, Chiavarina B, Zhou J, Wang C, Pavlides S, Martinez-Cantarin MP, Capozza F, Witkiewicz AK, Flomenberg N, Howell A, Pestell RG, Caro J, Lisanti MP, Sotgia F. Cell Cycle. 2010 Sep 1;9(17):3515-33. Epub 2010 Sep 9.
An RNA-directed nucleoside anti-metabolite, - An RNA-directed nucleoside anti-metabolite,
Lui VW, Lau CP, Cheung CS, Ho K, Ng MH, Cheng SH, Hong B, Tsao SW, Tsang CM, Lei KI, Yamasaki Y, Mita A, Chan AT. Biochem Pharmacol. 2010 Jun 15;79(12):1772-80.
The Warburg effect is genetically determined in inherited pheochromocytomas. - The Warburg effect is genetically determined in inherited pheochromocytomas.
Favier J, Briere JJ, Burnichon N, Riviere J, Vescovo L, Benit P, Giscos-Douriez I, De Reynies A, Bertherat J, Badoual C, Tissier F, Amar L, Libe R, Plouin PF, Jeunemaitre X, Rustin P, Gimenez-Roqueplo AP. PLoS One. 2009 Sep 18;4(9):e7094.