TIGAR

Gene Symbol TIGAR
Entrez Gene 57103
Alt Symbol C12orf5, FR2BP
Species Human
Gene Type protein-coding
Description TP53 induced glycolysis regulatory phosphatase
Other Description TP53-induced glycolysis and apoptosis regulator|fructose-2,6-bisphosphatase TIGAR|fructose-2,6-bisphosphate 2-phosphatase|probable fructose-2,6-bisphosphatase TIGAR|transactivated by NS3TP2 protein
Swissprots Q9NQ88 B2R840
Accessions EAW88849 EAW88850 Q9NQ88 AJ272206 CAC01127 AK313226 BAG36037 AM392615 CAL37493 AY425618 AAQ98969 BC012340 AAH12340 BX098281 DB480618 NM_020375 NP_065108
Function Fructose-bisphosphatase hydrolyzing fructose-2,6- bisphosphate as well as fructose-1,6-bisphosphate. Inhibits glycolysis by reducing cellular levels of fructose-2,6- bisphosphate. May protect cells against reactive oxygen species and against apoptosis induced by tp53. {ECO:0000269|PubMed:16839880}.
Top Pathways Central carbon metabolism in cancer, Fructose and mannose metabolism

TIGAR (D3F4A) Rabbit mAb - 14751 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3F4A
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with Doxorubicin #5927 (0.5 μM, 24 hr; +), using TIGAR (D3F4A) Rabbit mAb (upper), p53 (7F5) Rabbit mAb #2527 (middle) or β-Actin (13E5) Rabbit mAb #4970 (lower).

TIGAR (F-5) - sc-377065 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TIGAR (F-5): sc-377065. Western blot analysis of TIGAR expression in U-2 OS (A), Saos-2 (B) and Jurkat (C) whole cell lysates and human breast tissue extract (D).

TIGAR (G-2) - sc-74577 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TIGAR (G-2): sc-74577. Western blot analysis of TIGAR expression in U-2 OS (A), HeLa (B), Hep G2 (C) and KNRK (D) whole cell lysates.

TIGAR (H-120) - sc-67274 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TIGAR (H-120): sc-67274. Western blot analysis of TIGAR expression in U-2 OS (A), HeLa (B) and Hep G2 (C) whole cell lysates.

TIGAR (M-209) - sc-67273 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TIGAR (M-209): sc-67273. Western blot analysis of TIGAR expression in Saos-2 whole cell lysate.

TIGAR (M2-P4H2) - sc-101393 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

TIGAR (Y-20) - sc-68239 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TIGAR (Y-20): sc-68239. Western blot analysis of TIGAR expression in U-2 OS whole cell lysate.

TIGAR (E-2) - sc-166290 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TIGAR (E-2): sc-166290. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.

TIGAR (E-10) - sc-166291 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TIGAR (E-10): sc-166291. Western blot analysis of TIGAR expression in U-2 OS (A) and HeLa (B) whole cell lysates.

TIGAR (V-18) - sc-68238 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TIGAR (9C10) - sc-101535 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TIGAR (9C10): sc-101535. Western blot analysis of TIGAR expression in HeLa (A) and Hep G2 (B) whole cell lysates.

Anti-TIGAR antibody - ab37910 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TIGAR antibody (ab37910) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-TIGAR antibody - ab62533 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-TIGAR antibody (ab62533) at 0.5 µg/mlLane 2 : Anti-TIGAR antibody (ab62533) at 1 µg/mlLane 3 : Anti-TIGAR antibody (ab62533) at 2 µg/mlLane 1 : MCF7 cell lysateLane 2 : MCF7 cell lysateLane 3 : MCF7 cell lysateLysates/proteins at 15 µg per lane.

Anti-TIGAR antibody - ab189038 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling TIGAR with ab189038 at 5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-TIGAR antibody - ab189164 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Lanes 1 - 2 : Anti-TIGAR antibody (ab189164) at 1 µg/mlLane 3 : Anti-TIGAR antibody (ab189164) at 4 µg/mlLane 4 : Anti-TIGAR antibody (ab189164) at 2 µg/mlLane 1 : Human liver lysateLane 2 : Human liver lysate plus immunizing peptideLane 3 : Mouse liver lysateLane 4 : Rat liver lysate

Anti-TIGAR antibody - ab113980 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling TIGAR with ab113980 at 1/1000 (1µg/ml). Detection: DAB.

Anti-TIGAR antibody - ab197144 from Abcam

Prices $376.00
Sizes 250 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal kidney tissue labeling TIGAR with ab197144 at 1/100 dilution.

Anti-TIGAR antibody [9C10] - ab70468 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 9C10

Anti-TIGAR antibody [M2 - P4H2] - ab64622 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone M2 - P4H2
All lanes : Anti-TIGAR antibody [M2 - P4H2] (ab64622) at 10 µg/mlLane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Anti-TIGAR antibody [M2 - P4H2] (FITC) - ab150273 from Abcam

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone M2 - P4H2

Anti-TIGAR antibody [M2 - P4H2] - BSA and Azide free - ab174810 from Abcam

Prices $372.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone M2 - P4H2

Anti-TIGAR antibody - C-terminal - ab137573 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab137573 stained MCF7 cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab137573, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TIGAR antibody - N-terminal - ab174220 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-TIGAR antibody - N-terminal (ab174220) at 1/100 dilution + Mouse kidney tissue lysates at 35 µg

Anti-TIGAR Antibody (Internal) IHC-plus™ - LS-B1341 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-C12orf5 / TIGAR antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1341 concentration 5 ug/ml.

Anti-TIGAR Antibody (aa50-200, Biotin) - LS-C143216 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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