Anti-TIGAR antibody - C-terminal
| Name | Anti-TIGAR antibody - C-terminal |
|---|---|
| Supplier | Abcam |
| Catalog | ab137573 |
| Prices | $384.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide, corresponding to a region within the C terminal amino acids 206-270 of Human TIGAR (Uniprot ID Q9NQ88) |
| Description | Rabbit Polyclonal |
| Gene | TIGAR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab137573 stained MCF7 cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab137573, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-TIGAR antibody - C-terminal (ab137573) at 1/3000 dilutionLane 1 : Hep G2 whole cell lysateLane 2 : MOLT4 whole cell lysateLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of paraffin-embedded Hela xenograft labelled with ab137573 at 1/500 dilution.