Anti-TIMP3 antibody - Loop 1

• Supplier: Abcam
• Catalog: ab39184
• Prices: $395.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide of TIMP3 based on loop #1 of the human TIMP3
• Description: Rabbit Polyclonal
• Gene: TIMP3
• Conjugate: Unconjugated

Product images

All lanes : Anti-TIMP3 antibody - Loop 1 (ab39184)Lane 1 : Human TIMP3Lane 2 : Mouse TIMP3Lane 3 : crude control (preparation of extracellular matrix from BHK cells producing recombinant human TIMP-3)

ICC/IF image of ab39184 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39184, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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