All lanes : Anti-Tin2 antibody [EPR15319] (ab197894) at 1/10000 dilutionLane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysateLane 2 : WI38 (Human fetal lung fibroblast cells) whole cell lysateLane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Tin2 antibody [EPR15319] (ab197894) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Mouse kidney lysateLane 4 : Mouse spleen lysateLane 5 : C6 (Rat glial tumor cells) whole cell lysateLane 6 : RAW264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 7 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 8 : NIH3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Tin2 antibody [EPR15319] (ab197894) at 1/2000 dilutionLane 1 : Human thymus lysateLane 2 : Mouse thymus lysateLane 3 : Rat thymus lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution