All lanes : Anti-TIP120A antibody [EPR14241] - N-terminal (ab183748) at 1/20000 dilutionLane 1 : 293T whole cell lysateLane 2 : Jurkat whole cell lysateLane 3 : Saos-2 whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of Paraffin-embedded Human pancreas tissue labeling TIP120A with ab183748 at 1/100 dilution. HRP conjugated Rabbit IgG was used as a secondary antibody. Counter stained with Hematoxylin.
Flow cytometric analysis of 293T cells (fixed in 2% paraformaldehyde) labeling TIP120A with ab183748 at 1/220 dilution (red). Isotype control Rabbit monoclonal IgG (green). Secondary antibody Goat anti rabbit IgG (FITC) at a 1/150 dilution.
Western blot analysis of TIP120A in immunoprecipitation pellets from Jurkat lysate. ab183748 used at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.