All lanes : Anti-TIP120A antibody [EPR14242(B)] - C-terminal (ab181216) at 1/50000 dilutionLane 1 : 293T cell line lysateLane 2 : HeLa cell line lysateLane 3 : Jurkat cell line lysateLane 4 : Saos 2 cell line lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling TIP120A with ab181216 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot analysis of immunoprecipitation pellet from Hela cell lysate immunoprecipitated using ab181216 at 1/50 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling TIP120A with ab181216 at 1/20 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunofluorescent analysis of HeLa cells (-20℃ Acetone-fixed) labeling TIP120A with ab181216 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution and counterstained with Dapi (blue).