Immunohistochemistry of formalin-fixed, paraffin-embedded human small intestine tissue labeling TIP30 with ab192775 at 5 µg/ml.
Confocal Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling TIP30 using ab192775 at a 1/500 diluition (Green). Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1/2000.
All lanes : Anti-TIP30 antibody (ab192775) at 1/5000 dilutionLane 1 : A431 whole cell lysateLane 2 : HeLa whole cell lysateLysates/proteins at 30 µg per lane.
Western blot analysis of TIP30 in immunoprecipitation pellets from 40 µg HeLa whole cell lysate. ab192775 used at 1/1000 dilution (Lane 1). Negative control with 2 µg of preimmune rabbit IgG (Lane 2). Immunoprecipitation of TIP30 protein by 2 µg of TIP30 antibody (Lane 3). 10% SDS-PAGE.