Flow cytometric analysis of permeabilized HeLa cells labeling TIP30 using ab177961 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunofluorescence analysis of A431 cells labeling TIP30 using ab177961 at 1/50 dilution.
Immunohistochemical analysis of Human placenta tissue labeling TIP30 using ab177961 at 1/50 dilution.
Immunohistochemical analysis of Human pancreas tissue labeling TIP30 using ab177961 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab177961 at 1/10 dilution to immunoprecipitate TIP30, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes : Anti-TIP30 antibody [EPR13179] (ab177961) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : Fetal liver lysateLysates/proteins at 10 µg per lane.