All lanes : Anti-TIP30 antibody [EPR13185] (ab181378) at 1/10000 dilutionLane 1 : Human fetal liver tissue lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : A549 cell lysateLane 5 : SW480 cell lysateLysates/proteins at 10 µg per lane.
Western blot analysis on immunoprecipitation pellet from A431 cell lysate labeling TIP30 with ab181378 at 1/10 dilution (Lane 1). Lane 2 is 1XPBS (negative control). Detection with HRP-conjugated anti-rabbit IgG, detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized HeLa cells (red) labeling TIP30 with ab181378 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).