Anti-TIP49A antibody [2943C1a]
| Name | Anti-TIP49A antibody [2943C1a] |
|---|---|
| Supplier | Abcam |
| Catalog | ab51500 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 2943C1a |
| Applications | FC WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment, internal sequence |
| Description | Mouse Monoclonal |
| Gene | RUVBL1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-TIP49A antibody [2943C1a] (ab51500) at 1/100 dilution + HT-1080 whole cell lysate at 50 µgSecondaryAnti Mouse IgG at 1/2500 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/11408_ab51500.jpg)
Anti-TIP49A antibody [2943C1a] (ab51500) at 1/100 dilution + HT-1080 whole cell lysate at 50 µgSecondaryAnti Mouse IgG at 1/2500 dilution
Flow cytometry using ab51500 at 0.5ug using HeLa cells. Secondary used was an AlexaFluor® 488 conjugated Goat Anti-mouse IgG (0.25 µg). White peak shows an isotype control, while the red peak shows ab51500.
![All lanes : Anti-TIP49A antibody [2943C1a] (ab51500) at 1 µg/ml (in 3% BSA + PBS-Tween 0.1% for 12 hours at 4°C)Lane 1 : HeLa cell lysate + control siRNA sequenceLane 2 : HeLa cell lysate + control siRNA sequenceLane 3 : HeLa cell lysate suppressed by siRNA (1) sequence to pontin Lane 4 : HeLa cell lysate suppressed by siRNA (2) sequence to pontin Lane 5 : Cell lysate of 293T cellsLane 6 : Cell lysate of HeLa cellsLane 7 : Cell lysate of U251 cellsLane 8 : Cell lysate of Jurkat cellsLane 9 : Cell lysate of U2OS cellsLysates/proteins at 20 µg per lane.SecondaryAn HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/11410_TIP49A-Primary-antibodies-ab51500-2.jpg)
All lanes : Anti-TIP49A antibody [2943C1a] (ab51500) at 1 µg/ml (in 3% BSA + PBS-Tween 0.1% for 12 hours at 4°C)Lane 1 : HeLa cell lysate + control siRNA sequenceLane 2 : HeLa cell lysate + control siRNA sequenceLane 3 : HeLa cell lysate suppressed by siRNA (1) sequence to pontin Lane 4 : HeLa cell lysate suppressed by siRNA (2) sequence to pontin Lane 5 : Cell lysate of 293T cellsLane 6 : Cell lysate of HeLa cellsLane 7 : Cell lysate of U251 cellsLane 8 : Cell lysate of Jurkat cellsLane 9 : Cell lysate of U2OS cellsLysates/proteins at 20 µg per lane.SecondaryAn HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of ab51500 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51500, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![All lanes : Anti-TIP49A antibody [2943C1a] (ab51500) at 1/100 dilutionLane 1 : Whole cell lysate prepared from 9L cellsLane 2 : Whole cell lysate prepared from PC12 cellsLane 3 : Whole cell lysate prepared from RIE-1 cellsLysates/proteins at 20 µg per lane.SecondaryHRP conjugated goat anti-mouse polyclonal at 1/5000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/11412_TIP49A-Primary-antibodies-ab51500-9.jpg)
All lanes : Anti-TIP49A antibody [2943C1a] (ab51500) at 1/100 dilutionLane 1 : Whole cell lysate prepared from 9L cellsLane 2 : Whole cell lysate prepared from PC12 cellsLane 3 : Whole cell lysate prepared from RIE-1 cellsLysates/proteins at 20 µg per lane.SecondaryHRP conjugated goat anti-mouse polyclonal at 1/5000 dilutiondeveloped using the ECL technique
Product References
The human RVB complex is required for efficient transcription of type I - The human RVB complex is required for efficient transcription of type I
Gnatovskiy L, Mita P, Levy DE. Mol Cell Biol. 2013 Oct;33(19):3817-25.
Subunit organization of the human INO80 chromatin remodeling complex: an - Subunit organization of the human INO80 chromatin remodeling complex: an
Chen L, Cai Y, Jin J, Florens L, Swanson SK, Washburn MP, Conaway JW, Conaway RC. J Biol Chem. 2011 Apr 1;286(13):11283-9.
Tip60 is required for DNA interstrand cross-link repair in the Fanconi anemia - Tip60 is required for DNA interstrand cross-link repair in the Fanconi anemia
Hejna J, Holtorf M, Hines J, Mathewson L, Hemphill A, Al-Dhalimy M, Olson SB, Moses RE. J Biol Chem. 2008 Apr 11;283(15):9844-51.