ab57488 (1/200) detecting TIPIN in assynchronous HeLa cells (green). Cells were fixed in Paraformadehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.See Abreview
All lanes : Anti-TIPIN antibody (ab57488) at 1/500 dilutionLane 1 : 20 µg Human HCT116 (colorectal) whole cell lysateLane 2 : 20 µg Human HCT116 (colorectal) whole cell lysate treated with GAPD siRNA (3 days)Lane 3 : 20 µg Human HCT116 (colorectal) whole cell lysate treated with TIPIN siRNA (3 days)SecondaryGoat anti-Mouse conjugated to HRP at 1/10000 dilutionPerformed under reducing conditions.Observed band size : 48 kDa (why is the actual band size different from the predicted?)Additional bands at : 150 kDa (possible non-specific binding).This image is courtesy of an Abreview submitted by Dr Kirk McManusSee Abreview