All lanes : Anti-TLR9 antibody [EPR14964] (ab184943) at 1/5000 dilutionLane 1 : Ramos cell lysateLane 2 : Raji cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Anti-TLR9 antibody [EPR14964] (ab184943) at 1/1000 dilution + Daudi cell lysate at 20 µgSecondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunofluorescence analysis of, -20 °C acetone-fixed, Ramos cells labeling TLR9 with ab184943 at a 1/100 dilution. Secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.