Anti-TMEM173 antibody
| Name | Anti-TMEM173 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab92605 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human, Chimpanzee, Monkey, Dog |
| Antigen | Synthetic peptide corresponding to a region within amino acids 310 - 360 of Human TMEM173 (NP_938023) |
| Description | Rabbit Polyclonal |
| Gene | TMEM173 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-TMEM173 antibody (ab92605) at 1/1000 dilutionLane 1 : MCF7 whole cell lysateLane 2 : HepG2 whole cell lysateLysates/proteins at 25 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 42 kDa (why is the actual band size different from the predicted?)Additional bands at : 25 kDa (possible non-specific binding).Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview
Immunohistochemistry analysis of TMEM173 expression in formalin-fixed, paraffin-embedded Human breast cancer tissue an isotype control (1) or ab92605 at 5µg/ml (2 and 3).
ICC/IF image of ab92605 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92605, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Identification of a negative feedback loop between cyclic di-GMP-induced levels - Identification of a negative feedback loop between cyclic di-GMP-induced levels
Panchanathan R, Liu H, Xin D, Choubey D. Innate Immun. 2014 Oct;20(7):751-9.
Nuclear interferon-inducible protein 16 promotes silencing of herpesviral and - Nuclear interferon-inducible protein 16 promotes silencing of herpesviral and
Orzalli MH, Conwell SE, Berrios C, DeCaprio JA, Knipe DM. Proc Natl Acad Sci U S A. 2013 Nov 19;110(47):E4492-501. doi:
Nuclear IFI16 induction of IRF-3 signaling during herpesviral infection and - Nuclear IFI16 induction of IRF-3 signaling during herpesviral infection and
Orzalli MH, DeLuca NA, Knipe DM. Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):E3008-17. doi:
A role for DNA-dependent activator of interferon regulatory factor in the - A role for DNA-dependent activator of interferon regulatory factor in the
Furr SR, Chauhan VS, Moerdyk-Schauwecker MJ, Marriott I. J Neuroinflammation. 2011 Aug 12;8:99.