TMEM173

Gene Symbol TMEM173
Entrez Gene 340061
Alt Symbol ERIS, MITA, MPYS, NET23, SAVI, STING, hMITA, hSTING
Species Human
Gene Type protein-coding
Description transmembrane protein 173
Other Description N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner|endoplasmic reticulum IFN stimulator|endoplasmic reticulum interferon stimulator|mitochondrial mediator of IRF3 activation|stimulator of interferon genes protein
Swissprots D6RBX0 Q86WV6 A8K3P6 D6RID9 B6EB35 D6RE01
Accessions EAW62098 EAW62099 EAW62100 Q86WV6 AK095896 AK129800 AK290661 BAF83350 AX775913 CAE11656 BC047779 AAH47779 BE548954 BM149879 BM981219 BU731116 FJ222241 ACI46648 HQ448605 ADQ33083 KF430638 AHB86590 XM_005268445 XP_005268502 XM_011537639 XP_011535941 XM_011537640 XP_011535942 NM_001301738 NP_001288667 NM_198282 NP_938023
Function Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double- stranded DNA from viruses and bacteria delivered to the cytoplasm. Acts by recognizing and binding cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, and cyclic GMP-AMP (cGAMP), a messenger produced in response to DNA virus in the cytosol: upon binding of c-di-GMP or cGAMP, autoinhibition is alleviated and TMEM173/STING is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates d
Subcellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein. Cell membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Cytoplasm, perinuclear region. Cytoplasm. Note=In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited.
Tissue Specificity Ubiquitously expressed. Expressed in skin endothelial cells, alveolar type 2 pneumocytes, bronchial epithelium and alveolar macrophages. {ECO:0000269|PubMed:18724357, ECO:0000269|PubMed:18818105, ECO:0000269|PubMed:25029335}.
Top Pathways RIG-I-like receptor signaling pathway, Cytosolic DNA-sensing pathway

STING (D2P2F) Rabbit mAb - 13647 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2P2F
Western blot analysis of extracts from various cell lines using STING (D2P2F) Rabbit mAb.

STING Antibody - 3337 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

TMEM173 (E-20) - sc-241046 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TMEM173 (N-15) - sc-241044 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-TMEM173 antibody - ab124612 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab124612 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab124612 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TMEM173 antibody - ab179775 from Abcam

Prices $372.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-TMEM173 antibody - ab92605 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TMEM173 antibody (ab92605) at 1/1000 dilutionLane 1 : MCF7 whole cell lysateLane 2 : HepG2 whole cell lysateLysates/proteins at 25 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 42 kDa (why is the actual band size different from the predicted?)Additional bands at : 25 kDa (possible non-specific binding).Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview

Anti-TMEM173 antibody [2C9] - ab131573 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2C9
All lanes : Anti-TMEM173 antibody [2C9] (ab131573) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY TMEM173 cDNALysates/proteins at 5 µg per lane.

Anti-TMEM173 antibody [4E12] - ab131604 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4E12
All lanes : Anti-TMEM173 antibody [4E12] (ab131604) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA Lane 2 : HEK293T cells transfected with pCMV6-ENTRY TMEM173 cDNA Lysates/proteins at 5 µg per lane.SecondaryGoat anti-mouse-HRP at 1/5000 dilution

Anti-TMEM173 antibody [EPR13130] - ab181125 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13130
All lanes : Anti-TMEM173 antibody [EPR13130] (ab181125) at 1/2000 dilutionLane 1 : THP1 lysateLane 2 : HACAT lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-TMEM173 antibody [EPR13130] (Alexa Fluor® 488) - ab198950 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13130
ab198950 staining TMEM173 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198950 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-TMEM173 antibody [EPR13130] (Alexa Fluor® 647) - ab198952 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13130
ab198952 staining TMEM173 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198952 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLacells fixed with 4% formaldehyde (10 min).

Anti-TMEM173 antibody [EPR13130] (HRP) - ab198951 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13130
Anti-TMEM173 antibody [EPR13130] (HRP) (ab198951) at 1/5000 dilution + THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-TMEM173 antibody - C-terminal - ab189430 from Abcam

Prices $384.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-TMEM173 antibody - C-terminal (ab189430) at 1/1000 dilution + Mouse spleen tissue lysate at 35 µg

Anti-TMEM173 / STING Antibody (aa310-360) - LS-C148616 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TMEM173 / STING Antibody (aa216-265) - LS-C102283 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
TMEM173 antibody LS-C102283 Western blot of HeLa lysate.

Anti-TMEM173 / STING Antibody (aa268-317) - LS-C170298 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-TMEM173 / STING Antibody (aa311-340, APC) - LS-C241823 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TMEM173 / STING Antibody (aa311-340, Biotin) - LS-C241825 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TMEM173 / STING Antibody (aa311-340, PE) - LS-C241827 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TMEM173 / STING Antibody (aa311-340, FITC) - LS-C241826 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TMEM173 / STING Antibody (clone 4A7) - LS-C174098 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4A7
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM173 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM173.

Anti-TMEM173 / STING Antibody (aa311-340, AP) - LS-C241828 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TMEM173 / STING Antibody (aa311-340, APC) - LS-C241829 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TMEM173 / STING Antibody (aa311-340, FITC) - LS-C241830 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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