![All lanes : Anti-TMEM173 antibody [EPR13130] (ab181125) at 1/2000 dilutionLane 1 : THP1 lysateLane 2 : HACAT lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/12196_ab181125-213094-ab181125wb1.jpg)
All lanes : Anti-TMEM173 antibody [EPR13130] (ab181125) at 1/2000 dilutionLane 1 : THP1 lysateLane 2 : HACAT lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescence analysis of HACAT cells (fixative 4% paraformaldehyde) labeling TMEM173 with ab181125 at a 1/100 dilution, and counterstained with DAPI. Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.

Flow cytometry analysis of THP1 cells using ab181125 at a 1/10 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.