Anti-TMPRSS2 antibody [EPR3861]
| Name | Anti-TMPRSS2 antibody [EPR3861] |
|---|---|
| Supplier | Abcam |
| Catalog | ab92323 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3861 |
| Applications | WB IHC-P ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human, Monkey, Monkey, Marmoset |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TMPRSS2 aa 1-100 |
| Description | Rabbit Monoclonal |
| Gene | TMPRSS2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-TMPRSS2 antibody [EPR3861] (ab92323) at 1/1000 dilutionLane 1 : Human prostate lysateLane 2 : Human small intestine lysateLane 3 : LNCaP cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/12573_TMPRSS2-Primary-antibodies-ab92323-1.JPG)
All lanes : Anti-TMPRSS2 antibody [EPR3861] (ab92323) at 1/1000 dilutionLane 1 : Human prostate lysateLane 2 : Human small intestine lysateLane 3 : LNCaP cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/1000 dilution
ab92323 at 1/1000 dilution, staining TMPRSS2 in paraffin embedded Human prostatic adenocarcinoma
Overlay histogram showing LoVo cells stained with ab92323 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92323, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Transmembrane serine protease TMPRSS2 activates hepatitis C virus infection. - Transmembrane serine protease TMPRSS2 activates hepatitis C virus infection.
Esumi M, Ishibashi M, Yamaguchi H, Nakajima S, Tai Y, Kikuta S, Sugitani M, Takayama T, Tahara M, Takeda M, Wakita T. Hepatology. 2015 Feb;61(2):437-46.
Hypermethylation-mediated transcriptional repression of TMPRSS2 in androgen - Hypermethylation-mediated transcriptional repression of TMPRSS2 in androgen
Chu M, Chang Y, Wang N, Li W, Li P, Gao WQ. Exp Biol Med (Maywood). 2014 Apr 24;239(7):823-828.