Anti-TMPRSS2 antibody [EPR3862]

• Supplier: Abcam
• Catalog: ab109131
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3862
• Applications: ICC/IF ICC/IF WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TMPRSS2 aa 450-550 (C terminal)
• Description: Rabbit Monoclonal
• Gene: TMPRSS2
• Conjugate: Unconjugated

Product images

ab109131 staining TMPRSS2 in the HT-29 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

ab109131 staining TMPRSS2 in Human prostrate adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/1000). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

All lanes : Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/5000 dilutionLane 1 : Human prostrate LysateLane 2 : LnCaP LysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/1000 dilution (unpurified)Lane 1 : Human prostate tissue lysateLane 2 : Human prostate tissue lysateLysates/proteins at 10 µg per lane.

Immunohistochemical analysis of paraffin-embedded Human prostatic adenocarcinoma using ab109131, unpurified, at a dilution of 1/500.