All lanes : Anti-TMPRSS4 antibody (ab133954) at 1 µg/mlLane 1 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)Lane 2 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell LysateLane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell LysateLane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLane 5 : HCT 116 (Human Colorectal Carcinoma) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.