Anti-TNF beta antibody [AT15A3]
| Name | Anti-TNF beta antibody [AT15A3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab100844 |
| Prices | $384.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | AT15A3 |
| Applications | IHC-P WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment, corresponding to amino acids 35-205 of Human TNF beta (NP_001153212) |
| Description | Mouse Monoclonal |
| Gene | LTA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab100844 staining in human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab100844, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Anti-TNF beta antibody [AT15A3] (ab100844) at 1/1000 dilution + Mouse Spleen Tissue Lysate at 35 µgSecondarygoat anti-mouse conjugated to HRPdeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/12740_TNF-beta-Primary-antibodies-ab100844-1.jpg)
Anti-TNF beta antibody [AT15A3] (ab100844) at 1/1000 dilution + Mouse Spleen Tissue Lysate at 35 µgSecondarygoat anti-mouse conjugated to HRPdeveloped using the ECL technique
Immunofluorescence of Human HeLa cells stained with ab100844 at 1/500 with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).