Anti-TNIK (phospho S769) antibody
| Name | Anti-TNIK (phospho S769) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab60032 |
| Prices | $359.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | A KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S769 of human TNIK |
| Description | Rabbit Polyclonal |
| Gene | TNIK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ab60032 at a dilution of 1/50. A peroxidase-conjugated secondary antibody was used, followed by AEC staining.
ICC/IF image of ab60032 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60032, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.