Anti-Tollip antibody
| Name | Anti-Tollip antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab37155 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ELISA WB ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to 16 amino acids near to the C terminus of the human Tollip protein |
| Blocking Peptide | Active human Tollip peptide |
| Description | Rabbit Polyclonal |
| Gene | TOLLIP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-Tollip antibody (ab37155) at 1 µg/mlLane 2 : Anti-Tollip antibody (ab37155) at 2 µg/mlLane 1 : Rat brain cell lysateLane 2 : Rat brain cell lysate
ab37155 at 2 µg/ml staining Tollip in THP-1 cells by ICC. Secondary antibody was biotin conjugated donkey anti-rabbit.
ab37155 staining Tollip in human placenta.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.