Anti-Topoisomerase I antibody
| Name | Anti-Topoisomerase I antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28432 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF ELISA |
| Species Reactivities | Human |
| Antigen | Recombinant full length Topoisomerase I protein (Human) |
| Description | Rabbit Polyclonal |
| Gene | TOP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab28432 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.TextText
ICC/IF image of ab28432 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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A rapid and sensitive assay for DNA-protein covalent complexes in living cells. - A rapid and sensitive assay for DNA-protein covalent complexes in living cells.
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Development and validation of an immunoassay for quantification of topoisomerase - Development and validation of an immunoassay for quantification of topoisomerase
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Poly(ADP-ribose) polymerase signaling of topoisomerase 1-dependent DNA damage in - Poly(ADP-ribose) polymerase signaling of topoisomerase 1-dependent DNA damage in
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Topoisomerase I levels in the NCI-60 cancer cell line panel determined by - Topoisomerase I levels in the NCI-60 cancer cell line panel determined by
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Dynamic proteomics of individual cancer cells in response to a drug. - Dynamic proteomics of individual cancer cells in response to a drug.
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