TOP1

Gene Symbol TOP1
Entrez Gene 7150
Alt Symbol TOPI
Species Human
Gene Type protein-coding
Description topoisomerase (DNA) I
Other Description DNA topoisomerase 1|type I DNA topoisomerase
Swissprots E1P5W3 O43256 A8KA78 Q9UJN0 Q12856 Q12855 Q15610 P11387 Q5TFY3
Accessions AAA61206 CAA36834 EAW75994 EAW75995 EAW75996 EAW75997 P11387 AK225095 AK292943 BAF85632 AK310508 AK310516 BC000943 AAH00943 BC004475 AAH04475 BC039009 BC056681 BC067349 AAH67349 BC136297 AAI36298 J03250 AAA61207 M27913 AAA61208 U07804 AAB60379 U07806 AAB60380 X16479 CAA34500 XM_011529032 XP_011527334 XM_011529033 XP_011527335 NM_003286 NP_003277
Function Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on
Subcellular Location Nucleus, nucleolus {ECO:0000269|PubMed:12149243}. Nucleus, nucleoplasm {ECO:0000269|PubMed:12149243}. Note=Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
Tissue Specificity Endothelial cells. {ECO:0000269|PubMed:19168442}.

Topo I (H-5) - sc-271285 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Topo I (H-5): sc-271285. Western blot analysis of Topo I expression in Ramos (A) and HeLa (B) whole cell lysates.

Topo I (C-15) - sc-5342 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Topo I (C-15): sc-5342. Western blot analysis of Topo I expression in K-562 nuclear extract.

Topo I (C-21) - sc-32736 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Topo I (C-21): sc-32736. Western blot analysis of Topo I expression in K-562 (A) and HeLa (B) nuclear extracts.

Topo I (H-300) - sc-10783 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Topo I (H-300): sc-10783. Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach cancer tissue showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Anti-Topoisomerase I antibody - ab85038 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Topoisomerase I antibody (ab85038) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Topoisomerase I antibody - ab28432 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab28432 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.TextText

Anti-Topoisomerase I antibody - ab58313 from Abcam

Prices $382.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Topoisomerase I antibody (ab58313) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human hepatocellular carcinoma.

Anti-Topoisomerase I antibody - ab93722 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab93722 at 1/100 dilution staining Topoisomerase I in Human Breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

Anti-Topoisomerase I antibody [1291CT875142166] - N-terminal - ab188380 from Abcam

Prices $376.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
Clone 1291CT875142166
All lanes : Anti-Topoisomerase I antibody [1291CT875142166] - N-terminal (ab188380) at 1/1000 dilutionLane 1 : HUVEC cell lysateLane 2 : Jurkat cell lysateLane 3 : MCF7 cell lysateLane 4 : rat PC12 cell lysateLysates/proteins at 35 µg per lane.Secondarygoat anti-mouse IgG H&L at 1/5000 dilutiondeveloped using the ECL technique

Anti-Topoisomerase I antibody [EPR5375] - ab109374 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5375
Overlay histogram showing HepG2 cells stained with ab109374 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109374, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Topoisomerase I antibody [EPR5375] (Alexa Fluor® 488) - ab197505 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5375
ab197505 staining Topoisomerase I in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197505 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in T47D cells fixed with 100% methanol (5 min).

Anti-Topoisomerase I antibody [EPR5375] (Alexa Fluor® 647) - ab196930 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5375
Overlay histogram showing T47D cells stained with ab196930 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196930, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.This antibody gave a positive signal in T47D fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Topoisomerase I antibody [EPR5375] (HRP) - ab196642 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5375
All lanes : Anti-Topoisomerase I antibody [EPR5375] (HRP) (ab196642) at 1/5000 dilutionLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell LysateLane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Topoisomerase I antibody [EPR5376(2)] - ab131166 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5376(2)
All lanes : Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : Jurkat cell lysateLane 3 : SW480 cell lysateLane 4 : K562 cell lysateLane 5 : Ramos cell lysateLane 6 : RAW264.7 cell lysateLane 7 : PC12 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit IgG at 1/2000 dilution

Anti-Topoisomerase I antibody [Mab1] - ab12407 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone Mab1

Anti-Topoisomerase I antibody - ChIP Grade - ab3825 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab3825 staining Topoisomerase I in human 293T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview

Anti-TOP1 / Topoisomerase I Antibody - LS-C39022 from LifeSpan Bioscience

Prices $335.00
Sizes 2 ml
Host Human
Clonality Polyclonal

Anti-TOP1 / Topoisomerase I Antibody (aa716-726, clone 23B11) - LS-C39755 from LifeSpan Bioscience

Prices $475.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 23B11

Anti-TOP1 / Topoisomerase I Antibody - LS-C39756 from LifeSpan Bioscience

Prices $415.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-TOP1 / Topoisomerase I Antibody - LS-C39758 from LifeSpan Bioscience

Prices $315.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-TOP1 / Topoisomerase I Antibody - LS-C90610 from LifeSpan Bioscience

Prices $485.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal

Anti-TOP1 / Topoisomerase I Antibody - LS-C108432 from LifeSpan Bioscience

Prices $240.00, $360.00, $530.00
Sizes 100 µl, 500 µl, 1 ml
Host Rabbit
Clonality Polyclonal
Human, Breast carcinoma: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-TOP1 / Topoisomerase I Antibody - LS-C132023 from LifeSpan Bioscience

Prices $395.00
Sizes 2 ml
Host Human
Clonality Polyclonal

Anti-TOP1 / Topoisomerase I Antibody (Trp412) - LS-C193277 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TOP1 / Topoisomerase I Antibody (aa2-31, APC) - LS-C271946 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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