Anti-Topoisomerase I antibody [EPR5375]
| Name | Anti-Topoisomerase I antibody [EPR5375] |
|---|---|
| Supplier | Abcam |
| Catalog | ab109374 |
| Prices | $392.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR5375 |
| Applications | WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to residues at the N-terminus of Human Topoisomerase I |
| Description | Rabbit Monoclonal |
| Gene | TOP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing HepG2 cells stained with ab109374 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109374, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-Topoisomerase I antibody [EPR5375] (ab109374) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : MCF7 cell lysateLane 3 : SW480 cell lysateLane 4 : K562 cell lysateLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/13176_Topoisomerase-I-Primary-antibodies-ab109374-5.JPG)
All lanes : Anti-Topoisomerase I antibody [EPR5375] (ab109374) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : MCF7 cell lysateLane 3 : SW480 cell lysateLane 4 : K562 cell lysateLysates/proteins at 10 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab109374 at a dilution of 1/100.
Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue using ab109374 at a dilution of 1/100.
Immunofluorescent staining of MCF7 cells using ab109374 at a dilution of 1/100.
Product References
R-loops associated with triplet repeat expansions promote gene silencing in - R-loops associated with triplet repeat expansions promote gene silencing in
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RhoB promotes gammaH2AX dephosphorylation and DNA double-strand break repair. - RhoB promotes gammaH2AX dephosphorylation and DNA double-strand break repair.
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Transcription-dependent dynamic supercoiling is a short-range genomic force. - Transcription-dependent dynamic supercoiling is a short-range genomic force.
Kouzine F, Gupta A, Baranello L, Wojtowicz D, Ben-Aissa K, Liu J, Przytycka TM, Levens D. Nat Struct Mol Biol. 2013 Mar;20(3):396-403.
Rescue of the genetically engineered Cul4b mutant mouse as a potential model for - Rescue of the genetically engineered Cul4b mutant mouse as a potential model for
Chen CY, Tsai MS, Lin CY, Yu IS, Chen YT, Lin SR, Juan LW, Chen YT, Hsu HM, Lee LJ, Lin SW. Hum Mol Genet. 2012 Oct 1;21(19):4270-85.