All lanes : Anti-TPM4 antibody [EPR13316] (ab181085) at 1/5000 dilutionLane 1 : HeLa whole cell lysateLane 2 : A549 whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling TPM4 with ab181085 at a 1/250 dilution.
Anti-TPM4 antibody [EPR13316] (ab181085) at 1/20000 dilution + C2C12 whole cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Western blot analysis on immunoprecipitation pellet from C2C12 cell lysate labeling TPM4 with ab181085. Right hand lane is the negative control.