Overlay histogram showing MOLT4 cells stained with ab109003 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109003, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-TPOR antibody [EPR4194] (ab109003) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : A431 cell lysateLane 3 : Human marrow lysateLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/13476_TPOR-Primary-antibodies-ab109003-2.JPG)
All lanes : Anti-TPOR antibody [EPR4194] (ab109003) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : A431 cell lysateLane 3 : Human marrow lysateLysates/proteins at 10 µg per lane.