Anti-TPR antibody
| Name | Anti-TPR antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58344 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: MAAVLQQVLE RTELNKLPKS VQNKLEKFLA DQQSEIDGLK GRHEKFKVES EQQYFEIEKR LSHSQERLVN ETRECQSLRL ELEKLNNQLK ALTEKNKE, corresponding to amino acids 1-99 of Human TPR Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | TPR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
TPR antibody (ab58344) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human cerebellum.
Immunofluorescence analysis of HEK293T cells, 48 hours after transfection with non-specific siRNA (upper panels) or TPR siRNA (lower panels). TPR was stained using ab58344 (green). Nuclei were stained using DAPI (blue).
![Overlay histogram showing HeLa cells stained with ab58344 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58344, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/13503_TPR-Primary-antibodies-ab58344-4.jpg)
Overlay histogram showing HeLa cells stained with ab58344 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58344, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Localization of nucleoporin Tpr to the nuclear pore complex is essential for Tpr - Localization of nucleoporin Tpr to the nuclear pore complex is essential for Tpr
Rajanala K, Nandicoori VK. PLoS One. 2012;7(1):e29921.
Heart failure induces significant changes in nuclear pore complex of human - Heart failure induces significant changes in nuclear pore complex of human
Tarazon E, Rivera M, Rosello-Lleti E, Molina-Navarro MM, Sanchez-Lazaro IJ, Espana F, Montero JA, Lago F, Gonzalez-Juanatey JR, Portoles M. PLoS One. 2012;7(11):e48957.