Lane 1 : Anti-TRAF2 antibody (ab37118) at 0.5 µg/mlLane 2 : Anti-TRAF2 antibody (ab37118) at 1 µg/mlLane 3 : Anti-TRAF2 antibody (ab37118) at 2 µg/mlLane 1 : Mouse liver tissue lysateLane 2 : Mouse liver tissue lysateLane 3 : Mouse liver tissue lysateLysates/proteins at 15 µg per lane.Secondarygoat anti-rabbit HRP at 1/10000 dilution
ab37118 at 10µg/ml staining TRAF2 in mouse liver tissue by IHC
ICC/IF image of ab37118 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37118, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.