Anti-TRAF2 antibody [33A1293]
| Name | Anti-TRAF2 antibody [33A1293] |
|---|---|
| Supplier | Abcam |
| Catalog | ab12122 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 33A1293 |
| Applications | IHC-P FC WB |
| Species Reactivities | Human |
| Antigen | Fusion protein: HVKTCGKCRVPCRFHAIG , corresponding to amino acids 205-222 of Human TRAF2 |
| Description | Mouse Monoclonal |
| Gene | TRAF2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab12122 staining in human colonic carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12122, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Overlay histogram showing HeLa cells stained with ab12122 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12122, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/13683_TRAF2-Primary-antibodies-ab12122-3.jpg)
Overlay histogram showing HeLa cells stained with ab12122 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12122, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
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The UL144 gene product of human cytomegalovirus activates NFkappaB via a - The UL144 gene product of human cytomegalovirus activates NFkappaB via a
Poole E, King CA, Sinclair JH, Alcami A. EMBO J. 2006 Sep 20;25(18):4390-9. Epub 2006 Aug 24.