All lanes : Anti-TRAF6 antibody (ab137452) at 1/1000 dilutionLane 1 : A549 whole cell lysateLane 2 : HeLa whole cell lysateLane 3 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.
All lanes : Anti-TRAF6 antibody (ab137452) at 1/1000 dilutionLane 1 : NIH 3T3 whole cell lysateLane 2 : BCL-1 whole cell lysateLysates/proteins at 30 µg per lane.
Immunofluorescence analysis of methanol-fixed HeLa cells, labelling TRAF6 using ab137452 at 1/200 dilution. Right panel costained with Hoerchst 33342.
Immunohistochemical analysis of paraffin embedded 59T xenograft tissue, labelling TRAF6 using ab137452 at 1/500 dilution.